2012•Journal of Beijing University of Traditional Chinese MedicineRequires access
Influence of eye acupuncture on expression of brain ICAM-1 in rats with acute cerebral ischemia and reperfusion injury
Guan Hong-quan
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Abstract
Objective To observe the influence of eye acupuncture on the expression of brain intercellular cell adhesion molecules-1(ICAM-1) in rats with cerebral ischemia and reperfusion injury for 24 hours,and discuss the effect mechanism of eye acupuncture to cerebral ischemia and reperfusion injury.Methods SD rats were randomly divided into blank group,sham-operation group,model group and eye acupuncture group.The rat model of cerebral ischemia and reperfusion was established by using suture method.The brain patholegical changes were observed with HE staining in rats of all groups.The expression of brain ICAM-1 was detected by using the methods of ELISA,immunohistochemistry,real-time fluorescence quantitative polymerase chain reaction(RT-PCR) and Western-blot method.Results Compared with blank group,the content and expression of brain ICAM-1 increased significantly in model group.Compared with model group,the content and expression of brain ICAM-1 decreased significantly in eye acupuncture group.Conclusion One of effective mechanism of eye acupuncture for treating cerebral ischemia and reperfusion injury may be related to down-regulation of ICAM-1 expression.