Expression of Q-Type Human Paraoxonase in Escherichia.coli

Objective: To express human paraoxonase(PON1) in E.coli expression system. Methods: PON1 gene was amplified by PCR from pBlueScript-PON1 recombinant plasmid, and then was cloned into pBV220 vector to construct pBV220-PON1 expression plasmid. pBV220-PON1 plasmid was transformed into compenant cells of E.coli, and an expression strain was selected. Results: SDS-PAGE analysis revealed that the human PON1 protein was highly expressed and accumulated up to above 18% of the total amount of bacterial proteins in the form of inclusion body after induction. The inclusion bodies which contained recombinant proteins were isolated, denatured and refolded, however the treated protein did not show any enzyme activities. Conclusion: rhPON1 was expressed in the form of inclusion body in E.coli, recombinant protein was highly expressed.