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2001•Journal of Modern RehabilitationRequires access

The study of outgrowth and differentiation effects of bFGF and EGF on embryonic neural stem cells

Yingmao Gao

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Abstract

Objective To study the effects of bFGF and EGF on the embryonic neural stem cells in primary culture.Method Embryonic E12 rats were abtained from anesthetized timed pregnant Sprague-Dawley rats. Neural stem cells were dissociated from embryonic neural tube.The bFGF and EGF were added either alone or in combination into the culture medium.Results EGF mainly affected the proliferation of neural stem cells and slightly promoted the outgrowth of the processes, but could not stimulated the development of the cell bodies; while bFGF could stimulated the development of the cell bodies and processes.The data of EGF+bFGF group were superior to that of bFGF or EGFgroup. Conclusion bFGF and EGF both can enhance the outgrowth and differentiation of neural stem cells in primary culture.

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Objective To study the effects of bFGF and EGF on the embryonic neural stem cells in primary culture.Method Embryonic E12 rats were abtained from anesthetized timed pregnant Sprague-Dawley rats. Neural stem cells were dissociated from embryonic neural tube.The bFGF and EGF were added either alone or in combination into the culture medium.Results EGF mainly affected the proliferation of neural stem cells and slightly promoted the outgrowth of the processes, but could not stimulated the development of the cell bodies; while bFGF could stimulated the development of the cell bodies and processes.The data of EGF+bFGF group were superior to that of bFGF or EGFgroup. Conclusion bFGF and EGF both can enhance the outgrowth and differentiation of neural stem cells in primary culture.

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Available abstract

Objective To study the effects of bFGF and EGF on the embryonic neural stem cells in primary culture.Method Embryonic E12 rats were abtained from anesthetized timed pregnant Sprague-Dawley rats. Neural stem cells were dissociated from embryonic neural tube.The bFGF and EGF were added either alone or in combination into the culture medium.Results EGF mainly affected the proliferation of neural stem cells and slightly promoted the outgrowth of the processes, but could not stimulated the development of the cell bodies; while bFGF could stimulated the development of the cell bodies and processes.The data of EGF+bFGF group were superior to that of bFGF or EGFgroup. Conclusion bFGF and EGF both can enhance the outgrowth and differentiation of neural stem cells in primary culture.

Key concepts: Embryonic stem cell, Neural stem cell, Basic fibroblast growth factor, Cell biology, Neural tube, Stem cell, Epidermal growth factor, Biology

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