2005•Xiandai yufang yixueRequires access
APPLICATION OF ENTIRELY AUTOMATED NUCLEIC ACID AMPLIFICATION TEST ON HBV DNA IN BLOOD SCREENING.
Weigang Zhu
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Abstract
Objective:To investigate and study Roche nucleic acid entirely automated extraction method on HBV DNA in blood sample pooled by sampling processor.Methods:Individual donor plasma samples serologically negative for HBV were pooled by STAR2000 according to the size 24,using entirely automated extraction method to extraction nucleic acid simultaneously,amplified and detected by Roche COBAS AMPLICOR system,evaluating the sensitivity,efficacy and anti-interference.Results:The 95% cutoff for HPLC HBV DNA test was 38.9 IU/ml,95% CI was between 21 and 323,20 mg/dl bilirubin,1 g/dl hemoglobin and medium lipid blood did not interferer with the results,8 of 16 512 donations were PCR for HBV DNA positive,the DNA positive rate was 0.049%.Conclusion:Entirely automated extraction method by MPLC can be applied in the blood screening.