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Study on Conditions of Spore Fermentation for Bacillus Subtilis ACCC-10619

Lin Yuan-shan

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Abstract

The optimal formula of culture media and fermentation conditions for Bacillus subtilis ACCC-10619 were determined by methods of single factor tests and orthogonal experiments.The results showed that the optimal conditions were as follows: wheat bran 3.0%,glucose 3.0%,ammonium sulfate 0.5%,manganese sulfate 0.05%,initial pH 7.0,cultured at 33 ℃ for 36 h with static time 12 h.The output amounts of spore reached 8.5×1013cfu/L under volume of 100 L expanding fermentation.It could provide reference for production applications of Bacillus subtilis microbial ecological agents.

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The optimal formula of culture media and fermentation conditions for Bacillus subtilis ACCC-10619 were determined by methods of single factor tests and orthogonal experiments.The results showed that the optimal conditions were as follows: wheat bran 3.0%,glucose 3.0%,ammonium sulfate 0.5%,manganese sulfate 0.05%,initial pH 7.0,cultured at 33 ℃ for 36 h with static time 12 h.The output amounts of spore reached 8.5×1013cfu/L under volume of 100 L expanding fermentation.It could provide reference for production applications of Bacillus subtilis microbial ecological agents.

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Available abstract

The optimal formula of culture media and fermentation conditions for Bacillus subtilis ACCC-10619 were determined by methods of single factor tests and orthogonal experiments.The results showed that the optimal conditions were as follows: wheat bran 3.0%,glucose 3.0%,ammonium sulfate 0.5%,manganese sulfate 0.05%,initial pH 7.0,cultured at 33 ℃ for 36 h with static time 12 h.The output amounts of spore reached 8.5×1013cfu/L under volume of 100 L expanding fermentation.It could provide reference for production applications of Bacillus subtilis microbial ecological agents.

Key concepts: Bacillus subtilis, Fermentation, Bran, Food science, Ammonium sulfate, Spore, Chemistry, Manganese

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