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2007•Progress in Veterinary MedicineRequires access

The Sequence Analysis the Surface Glycoprotein Genes of a Duck Origin Influenza virus Isolate

Shicheng He

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Abstract

In this study,an avian influenza viruses(AIV)was isolated from Hunan province,and it was identified to be H5N1.According to the published sequence of H5N1 HA and NA genes,two pairs of specific primers were synthesized.The whole genetic encoding of the isolated strain of HA and NA genes were about 1.7 kb and 1.4 kb nucleotide with a single opening frame.The homology analysis showed that the homology of HA genes between the isolate and twelve reference viruses was 95.9%-98.0%,the homology of NA genes was 87.0%-98.4%.The deduced analysis of HA genes splitting site suggested that the nucleotides contain characteristic sequence of splitting site of high pathogenic AIV strain.

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What this paper is about

In this study,an avian influenza viruses(AIV)was isolated from Hunan province,and it was identified to be H5N1.According to the published sequence of H5N1 HA and NA genes,two pairs of specific primers were synthesized.The whole genetic encoding of the isolated strain of HA and NA genes were about 1.7 kb and 1.4 kb nucleotide with a single opening frame.The homology analysis showed that the homology of HA genes between the isolate and twelve reference viruses was 95.9%-98.0%,the homology of NA genes was 87.0%-98.4%.The deduced analysis of HA genes splitting site suggested that the nucleotides contain characteristic sequence of splitting site of high pathogenic AIV strain.

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Available abstract

In this study,an avian influenza viruses(AIV)was isolated from Hunan province,and it was identified to be H5N1.According to the published sequence of H5N1 HA and NA genes,two pairs of specific primers were synthesized.The whole genetic encoding of the isolated strain of HA and NA genes were about 1.7 kb and 1.4 kb nucleotide with a single opening frame.The homology analysis showed that the homology of HA genes between the isolate and twelve reference viruses was 95.9%-98.0%,the homology of NA genes was 87.0%-98.4%.The deduced analysis of HA genes splitting site suggested that the nucleotides contain characteristic sequence of splitting site of high pathogenic AIV strain.

Key concepts: Homology (biology), Gene, Influenza A virus subtype H5N1, Biology, Sequence analysis, Genetics, Virology, Sequence homology

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