Impact of High Intensity Focused Ultrasound Therapy on Immunity in Transplanted H22 Hepatic Carcinoma

[Porpuse] To investigate the impact of high intensity focused ultrasound (HIFU) therapy on immunity in H22 transplanted hepatic carcinoma. [Methods] Ninety C57BL/6J mice were randomly divided into three groups. One week after tumor implantation, the mice underwent HIFU ablation or HIFU sham ablation for the implanted tumor. Those in control group were normal mice, without either tumor implantation or HIFU treatment. Two weeks after HIFU, peripheral blood of each mouse was collected to detect T lymphocyte and subset by fluorescein activated cell sortor (FACS). [Results] Two weeks after HIFU, the tumor mass were completely regressive in all HIFU treated mice; none regression in sham-HIFU treated mice. Compared to the values in either sham-HIFU group, there were a significant increase of CD3+, CD4+ level and CD4+/CD8+ ratios, and a significant decrease of CD8+ level in HIFU treated group and control group. But compared with the values in either HIFU treated group and control group, there were no significant difference of CD3+, CD4+, CD8+ level and CD4+/CD8+ ratios.[Conclusion] The tumor mass can be regressive and the immunity improves after HIFU treatment for implanted H22 hepatic carcinoma. HIFU improves the anti-tumor immunity.