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2010•Shanxi yiyao zazhiRequires access

Glumatic acid and neurocyte apoptosis

Limin Zhang

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Abstract

Objective To study the toxic effect of glumatic acid on nerve cell of Sprague-Dawley rat by injecting different concentration glumatic acid and to determine the degree of nerve cell apoptosis in different time by using immunohistochemistry technique.Methods Seting SD rat model and observing the toxic effect of glumatic acid on nerve cell at different time by using cross immunity immunohistochemistry technique in situ and TUNEL staining method.Results There was remarkably difference between glumatic acid group and the normal sodium group by counting the number of nerve cell apoptosis(P0.05).Conclusion Specified dosage of glumatic acid can cause nerve cell apoptosis.

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What this paper is about

Objective To study the toxic effect of glumatic acid on nerve cell of Sprague-Dawley rat by injecting different concentration glumatic acid and to determine the degree of nerve cell apoptosis in different time by using immunohistochemistry technique.Methods Seting SD rat model and observing the toxic effect of glumatic acid on nerve cell at different time by using cross immunity immunohistochemistry technique in situ and TUNEL staining method.Results There was remarkably difference between glumatic acid group and the normal sodium group by counting the number of nerve cell apoptosis(P0.05).Conclusion Specified dosage of glumatic acid can cause nerve cell apoptosis.

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Available abstract

Objective To study the toxic effect of glumatic acid on nerve cell of Sprague-Dawley rat by injecting different concentration glumatic acid and to determine the degree of nerve cell apoptosis in different time by using immunohistochemistry technique.Methods Seting SD rat model and observing the toxic effect of glumatic acid on nerve cell at different time by using cross immunity immunohistochemistry technique in situ and TUNEL staining method.Results There was remarkably difference between glumatic acid group and the normal sodium group by counting the number of nerve cell apoptosis(P0.05).Conclusion Specified dosage of glumatic acid can cause nerve cell apoptosis.

Key concepts: TUNEL assay, Apoptosis, Immunohistochemistry, Staining, Cell, Chemistry, Andrology, Molecular biology

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