The regulatory effects of miR-142-3p on inflammatory factors released from THP-1 cells

Objective To investigate the role of miR-142-3p on inflammatory factors released from THP-1 cells.Methods Human monocytes THP-1 cells were cultured in vitro.(1) THP-1 cells were cultured for 24 h with 0.5 μg/mL and 2.0 μg/mL lipopolysaccharides(LPS).(2) THP-1 cells were transfected with miR-142-3p mimic(100 nmol/L) by lipofectamine RNAi MAX for 48 h.(3) THP-1 cells were transfected with miR-142-3p inhibitor(100 nmol/L) by lipofectamine RNAi MAX for 24 h,and cultured by 0.5 μg/mL and 2.0 μg/mL LPS for 24 h.The concentrations of TNF-α,IL-6 and MCP-1 in the cultured supernatant were assessed by ELISA.Results(1) The concentrations of TNF-α,IL-6 and MCP-1 in THP-1 cells stimulated with LPS were significantly increased with dose-dependent manners.(2) The transfection of miR-142-3p mimic significantly increased the release of TNF-α,IL-6 and MCP-1.(3) However,the pre-treatment of miR-142-3p inhibitor reversed the LPS-induced release of IL-6 from THP-1 cells.Conclusion miR-142-3p may play an important role in the regulation on inflammatory factors released from THP-1 cells.