THE SCREENING OF MICROSATELLITES MOLECULAR MARKERS WITH REFERENCE TO ANTI-VIBRIO ANGUILLARUM OF TURBOT SCOPHTHALMUS MAXIMUS

We explored 133 pairs of microsatellite markers included in Gen Bank to obtain suitable molecular markers that closely associated with anti-Vibrio anguillarum for turbot(Scophthalmus maximus L.) breeding. The experimental turbot by intraperitoneal injection with Vibrio anguillarum were divided into two groups: susceptible group and resistant group. The differential bands in the two turbot groups were selected, from which the correlation between the upper anti-Vibrio anguillarum and the microsatellite loci were analyzed, and the genetic diversity compared. The results show that alleles of Sma-USC108 and Sma-USC141 at 215 bp and 200 bp respectively were extremely significantly correlated with anti-Vibrio anguillarum with the Pearson correlation value of 0.363 and 0.407. On the other hand, alleles of FF0911, Sma-USC62, and Sma-USC279 at 189 bp, 163 bp, and 236 bp, respectively, were extremely significantly negative-correlated with anti-Vibrio anguillarum, with the Pearson correlation values at –0.377, –0.364, and –0.363(P0.01), respectively. We verified the above result with a second trial and determined that Sma-USC141 and FF0911 is useful for further molecular assistant selection of anti-Vibrio anguillarum turbot. Comparative analysis showed that they two groups were in low genetic variation at a same-level of genetic diversity.