Study on the adjustment to Candida albicans vaginal mcro-ecosystem with Lactobacillus

Objective Used the methods of microscopic examination of vaginal secretions for evidence of yeast forms.Histopathology microscopy examination and vaginal micro-ecosystem culture,the alteration of Condida albicans with Lactobacillus was studied. Methods Six to eight weeks old mice were vaginal washed wih antibiotic,then inoculated intra-vaginal with 20 μl of a suspension of Candida albicans to make mouse vaginal Candida imbalance model.Sixty female rats were divided into 6 groups including model group,natural recovery group,anti-fungal etc .After 6 treatments carried out,the Condida and Lactobacillus were quantified by culture of vaginal lavage fluid and HE staining of vaginal tissue during experimental vaginal candida. Results The amounts of Lactobacillus in the vaginal of model mouse decreased significantly ( P 0.01).The typical symptoms such as vaginal swell,clumpish secretion and congestive vaginal mucous membrane was appeared in the vagina of model rats.White blood cell and Candida albicans could be found when the vaginal secretion examined by microscopy.After 10 days therapy treatment,the amount of Lactobacillus in the vagina of rats treated by Lactobacilus was increased and the amount of Candida albicans was decreased significantly.The inhibit of Lactobacillus to Candida albicans was stronger than that of Zhimeimacin. Conclusions It was confirmed that the adhere of Candida albicans to vaginal mucous membrane could be preented by the bacteria of Lactobacillus imported from outside the vagina.The vaginal micro-ecosystem could be adjusted and the vaginal mucous membrane could be mended by Lactobacillus .