Enhancing effect of mutant α-synuclein on neurite outgrowth of rat primary cultured neuron

Objective To investigate the effect of A53T and A30P on the neurite outgrowth of brain neurons and indicate its function,explore the mechanism of Parkinson's disease.Methods Neurons isolated from the neocortex of newborn Wistar rats were cultured and corresponding proteins were added into the culture medium to observe the effect of the protein on the neurite outgrowth of the neurons.A53T,A30P and α-synuclein were added into the culture system of neural cells,and the different promoting effect of neurite outgrowth was compared with that of control groups.Western blotting assays and immunofluorescence staining assay were used to confirm whether the result is special.Inverted phase contrast microscope,and photographs were applied to analyze the results with software.Results The mean neurite length of the neurons treated with α-Syn was significantly longer than that of A53T and A30P group neurons(P 0.05).There was no significant difference between A53T and A30P groups with control group(P 0.05).Increased concentration of α-synuclein could enhance this effect.The result of Western blotting and immunofluorescence staining assay showed that α-synuclein could enter the neural cell and enhance the neurite outgrowth.Blockade with monoclonal antibody 3D5 showed that the blocking of α-Syn could significantly inhibit the effect(P 0.05).Conclusion α-Synuclein can enhance neurite outgrowth of primary cultured neuron in a dosedependent manner.But its mutants A53T and A30P did not show such effect.