Detecting T cell′s functions by anti-CD28 mAb costimulation

Objective To find a reliable mtthod for detecting T cell′s functions induced by anti CD28 mAb costimulated.Methods The CD28 mAb costimulated with CD3 mAb or non characteristic mitogen PHA induced T lymphocyte transformation and cytokines (interleukin 2, 4 and interferon γ) production by means of the 3H TdR incorporation, bioassay, and ELISA method. Results Anti CD28 mAb did not induce PBL proliferation and cytokines production alone. The G.I of CD28 mAb costimulated with CD3 mAb or PHA (20.1±9.5,53.9±17.2) stimulating PBL proliferation were more significantly increased than that of CD3 mAb or PHA (10.6±4.9,22.0±7.2) used alone( P 0.05, P 0.001). CD28 mAb costimulated with CD3 mAb or PHA dramatically increased cytokine production for about 0.7～10 times, as compared to CD3mAb or PHA stimulation alone. The PBL activation stimulated with CD28 mAb costimulation was influenced by culture time and the concentration of CD28 mAb. The concentration of CD28 mAb costimulation in 0.1～10 μg/ml induced PBL proliferation.Conclusion The CD28 mAb costimulation may be useful for detecting T cells functions.