Construction of Eukaryotic Expressing Plasmid of Chicken Interleukin 15 and Its Expression in vitro

The complete gene fragment of Chicken interleukin 15(ChIL-15) was amplified from T lymphocytes in spleen stimulated by ConA for 20 h by RT-PCR technique.Connect ChIL-15 to cloning vector pMD18-T,and the recombinant cloning plasmids were sequenced and named pMD18T-ChIL-15.ChIL-15 gene was then sequenced and cloned into eukaryotic expressing vector pcDNA3.1(+).The recombinant expressing plasmid was identified and transfected into 293T cell in vitro with Ca3(PO4)2.30 h after transfection,the expression of ChIL-15 protein was successfully detected by indirect immunofluorescence assay(IFA) with mouse-anti ChIL-15 monoclonal antibody.The study paves the way for the further study of ChIL-15 as an adjuvant.