ASSESSMENT OF AN ANTI-CANCER BIOACTIVE PEPTIDE ON HUMAN TUMOR CELL AND POSSIBLE MECHANISMS

Objective:To investigating the effects of anti-cancer bioactive peptide(ACBP)obtained from the goat organs on different human tumour cell lines and exploring its possible mechanisms.Methods:Huaman gastric cancer cell lines(BGC-823,MGC-803)and human nasopharyngeal cancer cell lines(CNE)were grown in the media with different doses of ACBP.The effect of ACBP on these cell growth were monitored by MTT assay,flow cytometry(FCM)and apoptotic assay at different times.Results:ACBP could inhibit the proliferation of all three cell lines(38.3%,29.79%and 42.02%respectively at 20g/mL)(P0.01)in a dose dependent manner.The FCM showed that the majority of the three control group cells were in G0/G1 phase and S phase,but reduced in number in S phase and increased in G0/G1 phase in ACBP group after 24h(P0.01).The cell apoptosis results also indicated that the number of apoptosis cell(AV+/PI-)increased in the early stage in ACBP group,became obvious in 48h and 72h.Conclusions:ACBP could inhibit the proliferation of human tumour cell lines.The possible mechanisms are inducing tumor cells apoptosis and suppressing DNA synthesis.