Effects of SP1-siRNA on apoptosis and proliferation of glioblastoma U251 cells

Objective To explore the inhibitory effect of transcription factor specificity protein 1(SP1)-siRNA on the apoptosis and proliferation of glioblastoma U251cells.Methods SP1-siRNA was transfected into U251cells.The transfection efficacy of SP1-siRNA was examined with Western blot.After SP1-siRNA transfection,the apoptosis of U251cells was detected by flow cytometry,proliferation by CCK-8,servivin expression by Western-blot,and Caspase-3activity by Caspase-3 activity test box.Results Compared to the U251cells in blank and negative control groups,the apoptosis rate was higher and proliferation was lower in the U251cells transfected with SP1-siRNA(P0.05).Compared to the U251cells in blank control group,the expression of survivin was downregulated and Caspase-3activity was enhanced in SP1-siRNA-transfected U251cells(P0.05). Conclusion The SP1-siRNA can effectively suppress the apoptosis and proliferation of glioblastoma U251cells.