Gus Gene Expression in Transgenic Cotton Cell and Its Correlation with NPTII and Bt Genes

The plant cell transformation of cotton cultivars with plasmid containing Gus gene,NPTⅡ gene and Bt gene driven by CaMV 35S promoter was carried out by using Agrobacterium mediaum . The transgenic colli and leaf tissues of the putative transgenic cotton plants were treated with X glucuronide,and the transgenic tissue expressed the turned blue Gus gene.The results of Histochemical Gus assay suggested that the Gus gene was stably transmitted into the next generation cell in callus mitotic cycle. The Gus activity ,NPTⅡ and Bt activity of the progenies(R 1,R 2) of trasgenic cotton plants (R 0) showed the genetic correlation but partly coexpression,the expression of the three transgenes turn out to be heterogeneous.