Effects of Heavy Ion Radiation on Oocytes and Early Development of In Vitro Fertilization Eggs in Sheep

The current experiment was conducted to examine the effect of heavy ion radiation in vitro maturation of oocytes and early development of fertilization eggs of sheep. Using different doses of heavy ion irradiation on oocyte and semen,we observed the ratio of in vitro maturation of oocytes and cleavage morula blastocyst of early embryos after fertilization. The results show that 1. 0 and1. 5 Gy could improve significantly the ratio of in vitro oocyte maturation compared with control group( p 0. 01); early development of the embryo had a significant change after fertilization of semen and oocyte by heavy ion radiating. When irradiatied on semen,the ratio of cleavage and mulberry embryo of early embryos increased significantly by 0. 5and1. 0 Gy dose irradiating( p 0. 05),but the ratio of blastocyst had no significant change. After radiating by1. 5 Gy dose,the ratio of cleavage was significantly increased( p 0. 05),while the cleavage,mulberry embryo and blastocyst was significantly decreased when radiation was 2. 0 Gy dose( p 0. 01). When irradiatied on oocyte,the ratio of cleavage,mulberry and blastocyst increased significantly when radiation were 0. 5and1. 0 Gy dose irradiating( p 0. 05),and the ratio of cleavage and mulberry significantly reduced( p 0. 01) after irradiating by 2. 0 Gy,but the ratio of blastula development is zero after oocyte radiated by 2. 0 Gy dose. Our finding suggested that low doses of heavy ion radiation promoted in vitro fertilization obviously,the early embryonic development but high doses of radiation inhibited cell growth.