Effect of 1, 25-dihydroxyvitamin D3 on the growth of cultured rat glomerular mesangial cells

【Objective】To study the effect of 1, 25-dihydroxyvitamin D3 on the growth and cell cycle of cultured rat glomerular mesangial cells. 【Methods】The cultured rat glomerular mesangial cells were treated with different dose of 1, 25(OH)2D3 (0～10-6 mol/L) for different time, the effect of mesangial cells proliferation were mesured by MTT colorimetric assay and the cell cycles were mesured by flow cytometry. 【Results】After 48 h of 1, 25(OH)2D3 (0~10-6mol/L) incubation,the mesangial cells′ growth and proliferation were inhibited significantly (P 0.01) and showed dose-dependently. The inhibited effect of a certain concentration 1, 25(OH)2D3 (10-8 mol/L) to mesangial cells showed time-dependently in definite range of time.After 72 h treatment of different concentration 1, 25(OH)2D3, significant difference were found in the cell cycle progression in response to 1,25 (OH)2D3 from the G1 to the S phases and apoptotic peak when compaired with thoses in the control group, especailly in the 10-6mol/L (P 0.01), 10-8 mol/L(P 0.05) concentration. The G2/M phases had no significant changes(P 0.05). 【Conclutions】0~10-6 mol/L 25 (OH)2D3 can inhibited the growth and proliferation of mesangial cells and promote their apoptosis in a concentration-dependent and time-dependent manner.