Effect of PEG Pretreatment on Antioxidant Enzymes Activity under Salt Stress in Rice Seedlings

Liaoxing 1 rice seedlings were used as experimental material. Rice seedlings were first treated for 3 d with 10%PEG-6000, then rewatered for 3 d, and added 100 mmol/L Na Cl to medium for 3 d, for the purpose to determine leaf water content, MDA content, antioxidant enzyme SOD, POD, CAT and APX activity. Results showed that under the rice seedling after PEG pretreatment and salt stress treatment, MDA content was 1.30(U/mol·g Fw), and salt stress treatment's MDA content was 1.57(U/mol·g Fw), it showed that MDA content by PEG pretreatment was significantly reduced compared with that by salt stress(P0.05), which indicated that PEG pretreatment had less damage to the leaves of rice seedlings, and its relative water content, antioxidant enzyme SOD, CAT, POD and APX activities in leaves were higher than those by salt stress(P0.05). PEG pretreatment could enhance the salt tolerance of rice seedlings.