2008•He'nan nongye kexueRequires access
Construction of the Eukaryotic Expression Vector for the HN Gene of Newcastle Disease Virus Isolated from Luoyang
Hebing Liu
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Abstract
The HN gene of Newcastle disease virus(NDV) isolated from Luoyang was amplified by RT-PCR and cloned into the eukaryotic expression vector pcDNA3.The recombinants of pcDNA3-HN were further identified by PCR amplification and enzyme digestion.The results showed that the expression vector pcDNA3-HN was successfully constructed.