Compare the Relationship Between Hepatitis B Virus DNA Detected by Quantitative PCR and the HBV Serum Markers

Objective To study the relation between the quantity of HBV-DNA and hepatitis B virus markers in hepatitis B patients.Methods Hepatitis B virus DNA was detected by FQ-PCR and hepatitis B virus markers were detected by ELISA in 320 serum sample.Results The amount of HBV-DNA was different in 3 HBV immunological Markers groups of 320 patients with positive HBVM . 188 had positive serum HBV-DNA (positive rate 56.2%) The positive rate of HBV-DNA in patients with positive serum HBsAg,HBeAg and HBcAb was 98.6%,and the positive rate of HBV-DNA content greater than 10copies/ml was 64.5%. The positive rate of HBV-DNA in patients with positive HBsAg HBeAb and HBcAb was 37.5%and the the positive rate of HBV-DNA content greater than 10 copies/ml was 18.2%.Conclusion There is a significant correlation between HBeAg and HBV-DNA .The serum HBV-DNA level quantified by PCR is more helpful for detecting the replication and communicability of virus in hepatitis B patients.