2007•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

Adenovirus Mediated p53 Gene Therapy for Human Hepatocellular Carcinoma

Dongwan Hong

Abstract

Objective:To explore the potential use of adenovirus mediated p53 gene(Ad-p53)therapy for hepatocellular carcinoma.Methods:A human hepatocellular carcinoma cell line HepG2 was used.Recombinant adenovirus carrying wild-type p53 gene was transfected into HepG2 cells in vitro and injected into tumor nodules in vivo.The growth of HepG2 cells in vitro and established hepatocellular carcinoma nodules in nude mice was examined.Cell apoptosis was analysed by Annexin-V/PI labeling flow cytometry method.Results:Cell growth was greatly suppressed at≥125MOI.p53 transfection can increase HepG2 cells apoposis rate.In vivo studies,intratumoral injection ofAd-p53 significantly inhibited hepatocellular carcinoma implanted xenograft.Conclusion:Transfection of wild-type p53 gene via Ad-p53 is a potential approach to the therapy of hepatocellular carcinoma.

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Key concepts: Hepatocellular carcinoma, Transfection, Genetic enhancement, Cancer research, Apoptosis, Flow cytometry, In vivo, Carcinoma