Detecting CD34~+ cells and their subsets in donors for allo-PBSCT

Objective To detect and analyze CD34 + cells and their subsets of peripheral blood in donor for allo PBSCT by flow cytometry.Methods Flow cytometry and multi color McAbs were used to detect CD34 + cells and their subsets.Results In 151 samples, the percentage of the CD34 + cells in MNCs was (0.954± 0.466)%, and the calculation of absolute CD34 + cells was (3.55±2.41)×10 9/L. The calculation of absolute CD34 +CD38 - cells was (0.253±0.24)×10 9/L, which was 6.78% of CD34 + cells;the calculation of absolute CD34 +HLA DR cells was (0.273±0.310)×10 9/L, or 6.82% of CD34 + cells. There was no significant difference between the enumeration of the CD34 +CD38 - cells and that of the CD34 +HLA DR cells. With the increasing of collection times and the donor's age, the numbers of CD34 + cells decreased accordingly. The percentage and the absolute CD34 + cells decreased by 47% and 50% respectively in donors above 40 years old than in donors below 20. There is a difference between numbers of CD34 + cells male and female donors, 23% higher in men the letter.Conclusion Detecting and analysing CD34 + cells and their subsets by flow cytometry and multi color analysis can not only give us exact numbers of CD34 + cells, but show us the quality of the hematopoietic stem cells for allo PBSCT.