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Analysis of the frequency and hot spot of mitochondrial DNA oxidative damage by ligation mediated PCR

Liu Li, 庄志雄, 陈雯, 杨杏芬, 凌文华, 魏青, 邓丽霞

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Abstract

Objective To study the relationship between the common deletion and oxidative damage of mitochondrial DNA(mtDNA). Methods The ligation mediated PCR(LMPCR) was used to map the frequency and hot spot of oxidative damage in the 5' end breakpoint region(8071～8190,about 120 bp) among the common deletion in the heavy strand of mtDNA from rat liver BRL 3A cell line exposed to 50 mmol/L of hydrogen peroxide(H 2O 2). Results Two oxidative damage hot spots were located in 8181G and 8182G.The regions in 8108C and 8096～8101(6 bp) were also severely damaged.The hot spots mapped in this study were surprisingly not the break point(8118T) of the common deletion. Conclusion The break point of common deletion in mtDNA is not necessarily the hot spot of oxidative damage.However,two hot spots were found in DNA break region.

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What this paper is about

Objective To study the relationship between the common deletion and oxidative damage of mitochondrial DNA(mtDNA). Methods The ligation mediated PCR(LMPCR) was used to map the frequency and hot spot of oxidative damage in the 5' end breakpoint region(8071～8190,about 120 bp) among the common deletion in the heavy strand of mtDNA from rat liver BRL 3A cell line exposed to 50 mmol/L of hydrogen peroxide(H 2O 2). Results Two oxidative damage hot spots were located in 8181G and 8182G.The regions in 8108C and 8096～8101(6 bp) were also severely damaged.The hot spots mapped in this study were surprisingly not the break point(8118T) of the common deletion. Conclusion The break point of common deletion in mtDNA is not necessarily the hot spot of oxidative damage.However,two hot spots were found in DNA break region.

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Available abstract

Objective To study the relationship between the common deletion and oxidative damage of mitochondrial DNA(mtDNA). Methods The ligation mediated PCR(LMPCR) was used to map the frequency and hot spot of oxidative damage in the 5' end breakpoint region(8071～8190,about 120 bp) among the common deletion in the heavy strand of mtDNA from rat liver BRL 3A cell line exposed to 50 mmol/L of hydrogen peroxide(H 2O 2). Results Two oxidative damage hot spots were located in 8181G and 8182G.The regions in 8108C and 8096～8101(6 bp) were also severely damaged.The hot spots mapped in this study were surprisingly not the break point(8118T) of the common deletion. Conclusion The break point of common deletion in mtDNA is not necessarily the hot spot of oxidative damage.However,two hot spots were found in DNA break region.

Key concepts: Mitochondrial DNA, Hot spot (computer programming), Oxidative phosphorylation, Molecular biology, Oxidative damage, DNA, DNA damage, Ligation

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