Screening of siRNA on Bcl-2 and Its Effect on Proliferation of Human Gastric Cancer Cell Line Cell MGC-803

Objective:To screen small interfering RNA(siRNA) sequences with Bcl-2 mRNA and study the inhibitory effect of Bcl-2-targeted siRNA in human MGC-803 gastric cancer cells.Methods:Four siRNAs targeting Bcl-2 gene were designed and synthesized,and they were transfeted into MGC-803 cells via LipofectimineTM2000.The inhibitory effect of siRNAs on the mRNA expression of Bcl-2 in infected cells were detected by real-time PCR,and on the protein exspression of Bcl-2 were tested by Western Blotting.The effect of suppressing proliferation of MGC-803 cells were detected by CCK-8.Results:Compared with the experimental and controls,siBcl-2,one of four couples of siRNAs,had the best inhibitory effect of Bcl-2 mRNA and protein expression,and the inhibition ratios were 88% and 63% respectively.The significant inhibitory effect of siBcl-2 on the reproduction of MGC-803 cells was observed as determined by CCK-8 after 96 hours transfection.Conclusion:The present experiment had successfully screened a couple of synthetic siRNA Sequence to Bcl-2 mRNA.It provided an useful experimental evidence for gene therapy of gastric cancer.