Ultrasonic Condition Optimization for Anthocyanin Extraction from Purple Cabbage by Using Response Surface Methodology(RSM)
Biyu Yang
Abstract
Biyu Yang
Abstract
The aim was to optimize the ultrasonic extraction method for anthocyanins from purple cabbage [Brassica campestris L. ssp. chinensis(L.) Makino var. communis Tsen et Lee] leaves. Under the conditions of 420 W ultrasonic power, 40% ethanol and twice extraction, the interactive effects of four crucial parameters, including pH, ratio of solid to liquid, ultrasonic time and ultrasonic temperature, were studied by central composite(BoxBehnken) and response surface methodology(RSM). The results showed that the optimum process for anthocyanin extraction was 1:16 of solid to liquid with pH 2.76 extracted at 58℃ for 12 min under 420 W ultrasonic power. Under this condition, the extract yield of anthocyanins from purple cabbage leaves reached up to 4.11 mg g–1 DW.
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The aim was to optimize the ultrasonic extraction method for anthocyanins from purple cabbage [Brassica campestris L. ssp. chinensis(L.) Makino var. communis Tsen et Lee] leaves. Under the conditions of 420 W ultrasonic power, 40% ethanol and twice extraction, the interactive effects of four crucial parameters, including pH, ratio of solid to liquid, ultrasonic time and ultrasonic temperature, were studied by central composite(BoxBehnken) and response surface methodology(RSM). The results showed that the optimum process for anthocyanin extraction was 1:16 of solid to liquid with pH 2.76 extracted at 58℃ for 12 min under 420 W ultrasonic power. Under this condition, the extract yield of anthocyanins from purple cabbage leaves reached up to 4.11 mg g–1 DW.
Key concepts: Response surface methodology, Extraction (chemistry), Anthocyanin, Ultrasonic sensor, Central composite design, Brassica, Yield (engineering), Chemistry