Antimicrobial resistance analysis among 4551 clinical isolates from our hospital

Objective To investigate the resistance of clinical isolates from our hospital in 2005.Methods Antimicrobial susceptibility testing was carried out by means of Kirby-Bauer(KB),and the data were analyzed by WHONET 5.3 software.Results Of 4 551 clinical isolates,gram-negative bacilli and gram-positive cocci accounted for 66.2% and 33.8%,respectively.The detection rate of methicillin-resistant isolates in Staphylococcus aureus(MRSA)was 68.6% and 86.8% in coagulase negative Staphylococcus(MRCNS),neither vancomycin nor teicoplanin resistant isolate was found in Staphylococcus,Enterococcus faecalis of Enterococcus faecium.The prevalence of extended-spectrum β-lactamases(ESBLs)was 55.8% in Escherichia.coli and 43.5% in Klebsiella pneumoniae.Imipenem and meropenem were the most potent antimicrobial agents against Enterobacteriaceae(96%).The isolates of mutiple-drug-resistant Acinetobacter baumannii were serious,the lowest resistant rate was to cefoperazone-sulbactam(35.7%),and 66.1% of the isolates was resistant to imipenem and meropenem.The most active agent against Pseudomonas aerugionsa was amikacin(resistant rate 14.7%),followed by ceftazidime,cefepime,cefoperazone-sulbactum and aztreonam(23%~29%),The resistant rate to imipenem(33.4%)was higher than that to meropenem(25.2%).Minocycline was the most active agent to Stenotrophomonas maltophilia,Chryseobacterium meningosepticum and Burkholderia cepacia(10%).Conclusion The antimicrobial resistance of common pathogenic bacteria was serious in our hospital,especially A.baumannii should be seriously betaken.