Construction of Anti-carcinoma ScFv Immunotoxin Recombinant Plasmid and its Expression

Objectives To construct the recombinant plasmid that expresses anti-carcinoma ScFv immunotoxin.Methods By recombinant technique,the cDNA coding the anti-carcinoma ScFv antibody was recombined with the cDNA coding Pseudomonas areuginosa exotoxin to form the recombinanr plasmid.The E Coli.BL-21,after being transformed with the recombinant plasmid,was induced by IPTG to express the anti-carcinoma ScFv immumotoxin.Results The recombinantion of plasmid was identifide to be correct by endonuclease restriction analysis and the BL-21 that contain the recombinant plasmid abundantly expressed the immunotoxin by IPTG inducement.The size of the expressed protein is similar to that anticipated in MW.Conclusions The recombinant plasmid that can express the anti-carcinoma ScFv immunotoxin is successfully obtained.