CONSTRUCTION AND SEQUENCE DETERMINATION OF RECOMBINANT E.COLI MYCOBACTERIA SHUTTLE PLASMID OF MSA2 CODING GENE OF PLASMODIUM FALCIPARUM

Objective Construction and sequence determination of recombinant E.coli Mycobacteria shuttle plasmid of MSA2 coding gene of Plasmodium falciparum . Methods The MSA2 gene fragment was amplified by PCR. After purification, the gene fragment was ligated with E.coli Mycobacteria shuttle plasmid pBCG5.6 at polylinker. The recombinant plasmid pBCG5.6/MSA2 was transferred into E.coli DH5α. Positive clones were screened and identified by PCR method and digested with restriction enzyme. The sequence of the inserted MSA2 gene fragment was also determined. Results and Conclusion The recombinant shuttle plasmid of MSA2 gene fragment of P. falciparum was successfully constructed. That would make it convenient for the further research of BCG malaria vaccine.