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2003•Medical Journal of West ChinaRequires access

The multidrug resistance character of HL-60/ADM cell line

Junyu Lu

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Abstract

Objective To detect the multidrug resistance character of HL-60/ADM cell line.Methods HL-60/ADM cells with multidrug resistance induced by adriamycin (ADM) were included in the experimental group, and the sensitive HL-60 cells were used in the control guoup. The proliferation and the chemosensitivity of cells were studied by MTT assay method. The expression of multidrug resistance protein (MRP) on the membrane of cells was detected by immunocytochemistry. The expression of MRP mRNA was determined by semi-quantitive RT-PCR method.Result The IC 50 values, for ADM, MTZ, VCR and H, in HL-60/ADM cells were significantly higher than those in HL-60 cells (P0.01), and the values of resistance fold were all more than 20 times. HL-60/ADM cells expressed abundnat MRP and MRP mRNA, but HL-60 cells did not express. Conclusion HL-60/ADM cell line can be used for MDR research in vitro.

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Objective To detect the multidrug resistance character of HL-60/ADM cell line.Methods HL-60/ADM cells with multidrug resistance induced by adriamycin (ADM) were included in the experimental group, and the sensitive HL-60 cells were used in the control guoup. The proliferation and the chemosensitivity of cells were studied by MTT assay method. The expression of multidrug resistance protein (MRP) on the membrane of cells was detected by immunocytochemistry. The expression of MRP mRNA was determined by semi-quantitive RT-PCR method.Result The IC 50 values, for ADM, MTZ, VCR and H, in HL-60/ADM cells were significantly higher than those in HL-60 cells (P0.01), and the values of resistance fold were all more than 20 times. HL-60/ADM cells expressed abundnat MRP and MRP mRNA, but HL-60 cells did not express. Conclusion HL-60/ADM cell line can be used for MDR research in vitro.

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Available abstract

Objective To detect the multidrug resistance character of HL-60/ADM cell line.Methods HL-60/ADM cells with multidrug resistance induced by adriamycin (ADM) were included in the experimental group, and the sensitive HL-60 cells were used in the control guoup. The proliferation and the chemosensitivity of cells were studied by MTT assay method. The expression of multidrug resistance protein (MRP) on the membrane of cells was detected by immunocytochemistry. The expression of MRP mRNA was determined by semi-quantitive RT-PCR method.Result The IC 50 values, for ADM, MTZ, VCR and H, in HL-60/ADM cells were significantly higher than those in HL-60 cells (P0.01), and the values of resistance fold were all more than 20 times. HL-60/ADM cells expressed abundnat MRP and MRP mRNA, but HL-60 cells did not express. Conclusion HL-60/ADM cell line can be used for MDR research in vitro.

Key concepts: Multiple drug resistance, Immunocytochemistry, Medicine, In vitro, Cell culture, Molecular biology, MTT assay, Messenger RNA

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