Effect of folic acid,VB6 and VB12 on expression of vascular cell adhesion molecule-1 in cultured endothelial cells in vitro

Objective To observe whether folic acid(FA),VB6 and VB12 have effects of inhibiting vascular cell ahesion molecule-1(VCAM-1) expression in cultured endothlial cells in vitro.Methods Cultured rat aortic endothelial cells were inoculated in vitro and divided into five groups:control group,1 mmol/L homocysteine(Hcy) group,inhibiting group Ⅰ(1 mmol/L Hcy+5μmol/L FA),inhibiting group Ⅱ(1 mmol/L Hcy+5 μmol/L FA+5 nmol/L VB12),inhibiting group Ⅲ(1 mmol/L Hcy+5μmol/L FA+0.1 μmol/L VB6+5nmol/L VB12).The expression of VCAM-1 mRNA in rat aortic endothelial cella induced by 1 mmol/L Hcy for 6 hours was assayed by RT-PCR.Results The expression level of NF-κB protein and VCAM-1 mRNA was up-regulated in the cultured rat aortic endothelial cells induced by 1.0 mmol/L Hcy for 6hr and 24hr respectively,and the rate of endotheliocyte-monocyte adhesion increased significantly.The effects induced by Hcy could be inhibited significantly by FA,VB6 and VB12,and the inhibitory effects adding FA,VB6 and VB12 together were better than other treatments.Conclusion FA,VB6 and VB12 may alleviate the injuries induced by Hcy in cultured endothelial cells in vitro through inhibiting the expression of NF-κB protein and VCAM-1 mRNA and decreasing the rate of endotheliocyte-monocyte adhesion.