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2011•Journal of clinical and experimental medicineRequires access

A study of the effect of CCL21/CCR7 axis on VEGF-C expression in human lung adenocarcinoma A549 cells

Changting Liu

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Abstract

Objective To investigate the effects of CCL21/CCR7 axis on VEGF-C expression of human lung adenocarcinoma A549 cells and A549-CCR7 cells.Methods VEGF-C expression was detected using Real-Time RT-PCR and Western Blot in A549 cells and A549-CCR7 cells before and after incubation with CCL21.Results Under the influence of CCL21,the VEGF-C expression of the A549-CCR7 cells,in mRNA and protein levels,was significantly increased compared to that of A549 cells(P0.01).Conclusion These data suggest that the bind of CCL21 to its receptor CCR7 leads to the increase of the VEGF-C expression of A549 cells,and CCL21/CCR7 axis may play a role in lymph node metastasis of lung cancer.To further study the relationship between CCL21/CCR7 axis and VEGF-C may help to elucidate the mechanism of lymph node metastasis in human lung cancer.

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Objective To investigate the effects of CCL21/CCR7 axis on VEGF-C expression of human lung adenocarcinoma A549 cells and A549-CCR7 cells.Methods VEGF-C expression was detected using Real-Time RT-PCR and Western Blot in A549 cells and A549-CCR7 cells before and after incubation with CCL21.Results Under the influence of CCL21,the VEGF-C expression of the A549-CCR7 cells,in mRNA and protein levels,was significantly increased compared to that of A549 cells(P0.01).Conclusion These data suggest that the bind of CCL21 to its receptor CCR7 leads to the increase of the VEGF-C expression of A549 cells,and CCL21/CCR7 axis may play a role in lymph node metastasis of lung cancer.To further study the relationship between CCL21/CCR7 axis and VEGF-C may help to elucidate the mechanism of lymph node metastasis in human lung cancer.

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Available abstract

Objective To investigate the effects of CCL21/CCR7 axis on VEGF-C expression of human lung adenocarcinoma A549 cells and A549-CCR7 cells.Methods VEGF-C expression was detected using Real-Time RT-PCR and Western Blot in A549 cells and A549-CCR7 cells before and after incubation with CCL21.Results Under the influence of CCL21,the VEGF-C expression of the A549-CCR7 cells,in mRNA and protein levels,was significantly increased compared to that of A549 cells(P0.01).Conclusion These data suggest that the bind of CCL21 to its receptor CCR7 leads to the increase of the VEGF-C expression of A549 cells,and CCL21/CCR7 axis may play a role in lymph node metastasis of lung cancer.To further study the relationship between CCL21/CCR7 axis and VEGF-C may help to elucidate the mechanism of lymph node metastasis in human lung cancer.

Key concepts: C-C chemokine receptor type 7, A549 cell, Medicine, CCL21, Lung cancer, Cancer research, Adenocarcinoma, VEGF receptors

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