Pharmacokinetics of Tyroservatide in Beagle Dogs after Single iv Injection

To study the pharmacokinetics of Tyroservatide in Beagle dogs after single iv injection a RP-HPLC-UV method for determining Tyroservatide in blood was established. The concentration-time profile was described after single iv injection of Tyroservatide in Beagle dogs, and the pharmacokinetics parameters were calculated. The RP-HPLC-UV method doesn't have a good LOQ, which is 0.20μg/ml. The profile of Tyroservatide in Beagle dogs fits to one compartment model. The biological half-life of Tyroservatide at three dose levels,60,600 and 6000μg/kg, is (8.88±1.45)s, (9.00±1.70)s, (11.12±2.83)s, which don’t have significant difference between the three levels. The C10s and AUC are proportional to dose, which suggests that Tyroservatide has the pharmacokinetics linearity over the dose range from 60μg/kg to 6000μg/kg. The preliminary result suggests that tyroservatide has a linearity pharmacokinetics behavior in Beagle dogs after iv administration. Tyroservatide is rapidly eliminated in vivo, and the biological half-life is less than 20 seconds.