The Observation of Neurogenesis in the Subventricular Zone in Rats after Transient Fo- cal Cerebral Ischemia

Objective: To observe the regularity of transient focal cerebral ischemia and neurogenesis of subventricular zone. Methods: Use middle cerebral artery occlusion (MCAO)to make focal cerebral ischemia model. BrdU, the thymidine analog that can incorporated into the DNA during the S phase of the cell cycle. Investigate the numbers of neurogenesis in the SVZ at 1, 4, 7, 10, 14 days after transient focal cerebral ischemia. Results: There was a significant increase in the numbers of BrdU immunoreactive cells in sub-ventricular zone at 4 days and with a peak at 7 days, then the numbers started to decease, but were still more than normal. Conclusion: Transient focal cerebral ischemia may enhance the neurogenesis of the subventricular zone and suggest that the cerebral has self-renovated capacity.