Relationship between ERCC1,Bcl-2 expression and cisplatin intervention in human lung adenocarcinoma cell lines

AIM:To study the relationship between the expressions of ERCC1,Bcl-2 and the effect of cisplatin in human lung adenocarcinoma cell lines,which suggested that the effects of ERCC1,Bcl-2 in cisplatin resistance effect.METHODS:A549 and A549/DDP cell lines were collected in the study,then the resistance of A549/DDP cells was measured by MTT assay.The expressions of ERCC1,Bcl-2 mRNA and protein after different concentrations' and different time' intervention were measured by immunocytohistology SABC assay and RT-PCR respectively.RESULTS:After treating with 10 μg/mL cisplatin for 12 h,the expressions of ERCC1,Bcl-2 mRNA and protein were increased in A549 cells;With the extension of time,the expressions of ERCC1 and Bcl-2 mRNA were increased gradually,they reached the peak levels in 72 h group.After treating with 5 μg/mL cisplatin for 24 h,the expressions of ERCC1,Bcl-2 mRNA and protein was increased in A549 cells.With the increase of concentration,the expressions of ERCC1 and Bcl-2 mRNA were increased gradually,they reached the highest levels in 20 μg/mL group.Compared with the paremtal cells,the expressions of ERCC1 and Bcl-2 mRNA and protein were increased significantly in A549/DDP cells.CONCLUSION:With increasing concentrations of cisplatin and action time,the expressions of ERCC1,Bcl-2 mRNA and protein are increased in A549 cells,then speculates that ERCC1 and Bcl-2 may be involved in cisplatin acquired resistance.