Status of drug resistance and detection of the AmpC enzyme,ampC gene expression in non-fermenting gram-negative bacilli

Objective To investigate the status of the drug resistance and the distribution of the ampC gene,the detection of the AmpC enzyme in 83 non-fermenting gram-negative bacilli(NFGNB).Methods Microscan walkaway-4.0 was used to detect the susceptibility of antimicrobial agents against 83 clinical strains. AmpC gene was amplified by polymerase chain reaction (PCR). AmpC enzyme was examined by Cefoxitin in three dimensional test.Results Among the 83 strains, ampC genes were found in 53 strains by PCR.Twelve strains had produced AmpC enzyme accounted for 14.5%. The sensitivity rates of 83 strains to IPM,CIP,AK,TOB,FEP,CN,CAZ,SXT,PIP,CRO,ATM were: 78.3%,74.7%,63.9%,62.7%,60.2%,51.8%,38.6%,32.5%,32.5%, 24.1%,22.9%, respectively.Conclusion The durg resistant status of non-fermenting gram negative bacilli is severe. Clearing out the strains producing AmpC enzyme will be helpful in selection of antimicrobial agents in the treatment of hospital infection.