A PRELIMINARY STUDY OF MICROBIAL DIVERSITY OF THE SURFACE LAYER SEDIMENTS FROM THE EAST CHINA SEA

The environmental total DNAs were directly extracted from the surface layer sediment of the East China sea. The bacterial and archaeal 16S rDNA gene libraries were generated by using PCR and TA cloning. Phylogenetic results show that there are eight phyla in the bacteria domain: Proteobacteria (41.5%), Planctomycete (10.9%), Actinobacteria (8.9%), CFB group (7.9%), Acidobacteria, Verrucomicrobia, Nitrospira and Firmicutes, the phylum Proteobacteria is predominant. In the archaea domain, all clones were belonged to Crenarchaeota and no one Euryarchaeota clone was detected. There are four divisions in the Crenarchaeota kingdom: Marine Crenarchaeotic Group Ⅰ (MG Ⅰ, 93.6%), Miscellaneous Crenarchaeotic Group (MCG), Marine Benthic Group C (MBGC), Marine Benthic Group A (MBGA), and MG Ⅰ is the dominating group. DOTUR statistics of 16S rDNA gene sequences from the libraries indicating a high richness in the sediment microbial communities.