EFFECT OF SEVOFLURANE ON SPECIFIC COGNITIVE FUNCTIONS IN THE ELDERLY CEREBRAL BLOOD FLOWED DEFICITS RATS

[Objective] To investigate the effect of sevoflurane on specific cognitive functions in the elderly Cerebral blood flowed deficits rats in order to provide references for clinical drug use. [Methods] Twenty four Wistar rats were randomly divided into 4 groups, including control group (Ⅰ), 2% sevoflurane group (Ⅱ), 3% sevoflurance group (Ⅲ) and sevoflurane-ische-group (Ⅳ). Rats in the four groups were respectively administrated for 100 minutes per day with 0%, 2%, 3% and 2% sevoflurane, and 2L·min-1 O2+6L·min-1 N2, and administrated for five days. Rats in Ⅳ group were ligated left common carotid artery before a week of breathing mixed gas. The cognitive function was tested 4 time a day for 5 days by using Morris water maze. The animals were killed after testing by Morris water maze. mRNA expression of NR, NR1 and NR2B in the hippocampus were determined by reverse transcriptase–polymerase chain reaction (RT-PCR). [Results] ① The repeated analysis of variance showed that the latency period and swimming distance in Ⅰ-Ⅳ group had gradually shorter during the five days, and the latency period and the swimming distance were significantly shorter at the fifth day than those at the first day (P﹤0.05). ② One way analysis of variance showed that mRNA expression of NR2B in group Ⅳ had significant difference among the four groups (F = 7.79, P﹤0.001). [Conclusion] Sevoflurane had dose-dependent inhibited effect on cognitive function of elderly rats. Cerebral blood flow could influence sevoflurane on the cognitive functions in elderly rats. mRNA expression of NR2B in group Ⅳ was higher, and it had association with earlier cerebral blood flow deficits.