Effects of HIF-1αsilencing on expression of p27 and Ki67 in rat hepatoma cell line CBRH-7919

AIM: To study the effect of hypoxia-inducible factor 1α(HIF-1α) silencing on the expression of p27 and Ki67 in rat hepatoma cell line CBRH-7919 under hypoxia.METHODS: Hypoxic condition was induced by CoCl2 and the expression of HIF-1α was silenced by small interference RNA.HIF-1α-specific RNAi lentiviral vector was constructed.Real-time RT-PCR and Western blotting were used to detect the expression of HIF-1α at mRNA and protein levels in CBRH-7919 cells under hypoxia.The expression of p27 and Ki67 was observed by Western blotting after HIF-1α silencing was performed.The cell cycle of hepatoma cells was detected by flow cytometry.RESULTS: Under hypoxic condition,the expression of HIF-1α at mRNA and protein levels in CBRH-7919 cells increased significantly(P0.05).HIF-1α silencing significantly reduced the expression of Ki67 but increased the expression of p27(P0.05) in CBRH-7919 cells.In transfected cells,the number of cells in G0/G1 phase was much higher and that in S phase was much lower than those in the control cells.CONCLUSION: Hypoxia induces the expression of HIF-1α.HIF-1α silencing can regulate the proliferation of hepatoma cells through reducing the expression of Ki67 and increasing the expression of p27.