Impacting of Insulin and Prolactin on Mammary Gland Epithelial Cell Line

Mammary gland epithelial cells which have lactating function can be a cellular mode of research of mammary gland development and lactating regulation mechanism.In this paper we studied the lactating function of dairy goat mammary gland epithelial cell line.SDS-PAGE,testing of triglyceride content and HPLC were used for detection of total proteins,milk fat and lactose respectively reflected lactating function of both insulin and prolactin impacting on dairy goat mammary gland epithelial cells.Results show that dairy goat mammary gland epithelial cells can excrete milk fat,milk proteins and lactose cultured in DF12 fluid after adding EGF,IGF-1,ITS and FBS.Lactating function of mammary gland epithelial cells has no obvious change after 48 h treated by insluin.However,treatment of prolactin can obviously raise the level of secretion of milk proteins and lactose.A mammary gland epithelial cell line which has lactating function has been established.This result is convenient for the study of lactation mechanism of dairy goats,dairy cows and other economic animals.