2008•Di-Si Junyi Daxue xuebaoRequires access
Inhibition on growth of ovarian carcinoma cells by recombinant adenovirus carrying pten gene
Lin Guan
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Abstract
AIM:To observe the expression of pten in human highly metastatic ovarian carcinoma cell line HO-8910PM after in vitro infection and investigate the effects of pten gene on the proli-feration and metastatic ability of human ovarian carcinoma HO-8910PM cells.METHODS:Ad-pten was constructed through Ad-Easy adenovirus vector system and the expression of pten gene in HO-8910PM cells was determined by Western Blot.The transfection efficiency of Ad-pten was determined by fluorescence microscope.The inhibitive effect of Ad-pten on proliferation of HO-8910PM cells was determined by MTT assay.The morpho-logic changes of HO-8910PM cells transfected with Ad-pten were observed by light microscopy(HE staining).The metastatic ability was analyzed by cell scratching model in vitro.Hoechest33258 staining was used to study the cell apoptosis.RESULTS:The expression of PTEN protein in HO-8910PM cells was confirmed by Western Blot.The efficiency of adenovirus mediated Ad-pten gene transfer was above 90% when the multiplicities of infection(MOI)was 100.Exogenous pten gene significantly suppressed the growth and metastatic ability of HO-8910PM cells and induced the apoptosis of the cells.CONCLUSION:The constructed Ad-pten transfection system is highly efficient in transferring wild-type pten gene into human endometrial carcinoma cells.Ad-pten can strongly inhibit the proliferation and metastasis of the cells,and also induce the apoptosis of the cells.