Effect of omeprazole on pharmacodynamics of clopidogrel

OBJECTIVE:To observe the influence of omeprazole on the anti-thrombus effect of clopidogrel.METHODS:The experimental animals were randomly divided into three groups,control group,clopidogrel group,combination groupfomeprazole 40 or 80 mg/kg+ clopidogrel 10 mg/kg),control group(methyl cellulose 1 mL).4 h after treatment,we measured the platelet aggregation rate,the quality of the thrombus in the arterio-venous shunt thrombosis model,and evaluated the antithrombotic effect of clopidogrel in rats.RESULTS:According to the results of platelet aggregation,we determined ADP concentration to be 50 μmol/L,and the dosage of clopidogrel 10 mg/kg.Compared with the control group,clopidogrel group showed significant inhibition of platelet aggregation,decreased the weight of thrombus.Omeprazole+ clopidogrel group compared with clopidogrel group could significantly increase the rate of platelet aggregation(43.7%and 23.3%,respectively,P0.01),and enhanced the weight of thrombus(41.45 and 21.31 mg,respectively,P0.01).CONCLUSION:80 mg/kg omeprazole inhibited the antithrombotic effect of clopidogrel.