Expression and significance of nuclear factor-κB in gastric ischemia/reperfusion injury of rats

Objective To study the expression and significance of nuclear factor-κB(NF-κB) in gastric ischemia/reperfusion(GI/R) injury of rats.?Methods The GI/R model was established by clamping the celiac artery for 30 min and allowing reperfusion.Rats were divided into a sham operation group and an ischemia/reperfusion injury group,with the latter including subgroups terminated 0.5,1,3,24 hours after reperfusion respectively.The animals were finally sacrificed to investigate the gastric mucosal damage index and the mucosal cell NF-κB activity by immunohistochemistry and Western blot.?Results It was shown that NF-κB was expressed at a very low level in the sham group,but was upregulated after gastric ischemia/reperfusion,approaching its peak after 1 hour of reperfusion and then coming down.The results were in good agreement with the changes of the gastric mucosal damage index.?Conclusion NF-κB may play an important role in gastric ischemia/reperfusion injury in rats.