The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231

Objective:To investigate the effects of bone morphogenetic protein 9 on the proliferation, cell cycle and apoptosis of breast cancer cell line MDA-MB-231.Methods:The expression of BMP9 in high-metastatic cell line MDA-MB-231 and low-metastatic cell line MCF-7 were detected by Semi-quantitative PCR respectively,MDA-MB-231 were transfected with AdBMP9(as experimental groups) and AdGFP(as control groups) respectively,Then the ability of cell proliferation was detected by MTT,cell cycle and apoptosis were detected by flow cytometry.Results:The BMP9 mRNA was not detected in human breast cancer cell line MDA-MB-231.After transfection with AdBMP9,the MDA-MB-231 cells showed an inhibited proliferation,On the fifth day,the growth rate of experimental groups(0.886 0±0.053 2)was significantly lower than that of control groups(1.224 0±0.103 1)(P0.05);The results of flow cytometry showed that,BMP9 could induce the cell cycle arrest in MDA-MB-231,On the second day and third day after transfection,the cell number of G2/M phase increased significantly,which were 3.2 fold and 2.4 fold that of control groups respectively;It's also found that after 72h treatment,the apoptosis rate of experimental groups(31.55%±8.26%) was significantly higher than that of control group(3.80%±0.46%)(P0.05).Conclusion:These data demonstrated that BMP9 could inhibit the proliferation of MDA-MB-231 by blocking cell cycle and inducing apoptosis.