Effects of leukemia inhibitory factor on nerve regeneration in rat

Objective To investigate the leukemia inhibitory factor(LIF) in rat sciatic nerve regeneration after injury.Methods Sixty male and female SD rats were randomly divided into two groups of thirty.Experimental group,the removal of the left sciatic nerve with a silicone tube 10 mm socket,set to take over within the injected LIF 50 μL(0.5 μg);the control group,the removal of the left sciatic nerve 10 mm socket with a silicone tube,into the same amount of saline as control.After 4,8,12 weeks,respectively,for each group of 10 rats the sciatic nerve index,nerve electrophysiological testing,and then remove the silicone tube and the ipsilateral gastrocnemius nerve for neuromuscular histological examination.Results After 4,8,12 weeks of experimental and control,the number of regenerated nerve fibers,conduction velocity,myelin thickness and volume of skeletal muscle and other than in the control group improved significantly(P0.001),Experimental group,the recovery of lower limb muscle function well.Conclusion LIF can promote the regeneration of rat sciatic nerve,and there is muscle nutrition.