Callus Induction and Plantlet Regeneration of Viburnum rhytidophyllum Hemsl.

The young leaves of Viburnum rhytidophyllum were taken as explants, and MS was basic medium. The effects of different concentrations and combinations of plant growth regulators on callus induction, bud differentiation and root formation were studied. Tissue culture regeneration system for V. rhytidophyllum was established. The results showed that the best sterilization method for materials was to soak explants into 0.1% HgC12 solution for 12 min after pretreatment with 75% alcohol for 20 s; the best medium for callus formation was MS+6-BA 1.0 mg·L-1+NAA 0.25 mg·L-1+2,4-D 1.0 mg·L-1, callus induction rate reached 92%; the optimum bud differentiation medium was MS+6-BA 1.5 mg·L-1+NAA 0.2 mg·L-1 with the induction rate of 87.25%; the suitable medium for root induction was 1/2MS+NAA 1.0 mg·L-1 and the rooting rate was 85%.