2004•Di-Si Junyi Daxue xuebaoRequires access
Protective effect of ischemic preconditioning on rat with acute renal ischemia/reperfusion injury and its roles in cell apoptosis
Hua Tan
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Abstract
AIM: To investigate the protective effect of ischemic preconditioning (IPC) on rats with acute renal ischemia/reperfusion (I/R) injury, and its effects on cell apoptosis and expression of Bcl 2 and Bax in the cells, and the possible mechanism of renal protection affected by IPC against I/R injury. METHODS: Fifty Wister rats of either sex were randomly divided into 5 groups: Group A (control group) in which sham operation was performed; group B (I/R group), in which ischemia was made by tying both renal arteries for 45 min, followed by 24 hour reperfusion; group C (IPC1 group) in which both renal arteries were first tied for 10 min then untied for 10 min, then followed the procedures as in group B; group D (IPC2 group) in which both renal arteries were first tied for 10 min then untied for 10 min and the process was repeated 2 times before the procedures in group B; group E (IPC3 group) in which both renal arteries were first tied for 10 min then untied for 10 min and the process was repeated 3 times before the procedures in group B. Apoptosis was examined by means of terminal deoxynucleotidyl transferase mediated d UTP nick end labeling (TUNEL). The expression of Bcl 2 and Bax protein was measured by immunohistochemical technique. RESULTS: Compared with those in the control group, the apoptotic index (3.1±2.3 vs 28.8±4.4, P 0.05) and the expression of Bax (183.0±12.8 vs 163.0± 17.1 , P 0.05) and Bcl 2 (184.0±9.6 vs 179.0±13.0, P 0.05)were higher, and the Bcl 2/Bax ratio decreased significantly(1.00±0.08 vs 1.10±0.07, P 0.05)in I/R group. Compared with those in the I/R group, the apoptotic index (28.8±4.4 vs 15.6±3.8, P 0.05) and the levels of expression of Bax decreased(163.0±17.1 vs 174.0±13.7, P 0.05), and the expression of Bcl 2 (179.0±13.0 vs 170.0±15.1, P 0.05) and the Bcl 2/Bax ratio increased (1.10±0.07 vs 0.98±0.11, P 0.05)in IPC3 group. CONCLUSION: IPC3 can protect kidneys from I/R injury, partly through the inhibition of apoptosis by regulating Bcl 2 and Bax expression.