Study on the haploid frequency of male sterile lines with Ae.bicornis cytoplasm in wheat

The four male sterile lines of wheat with Ae.bicornis cytoplasm,among them ms(bicor)-83(37)65,ms(bicor)-Yanshi No 9 and ms(bicor)-80(6)are 1B/1R genotype and ms(bicor)-90-110 is non-1B/1R genotype,were tested crossing with the aneuploid with different construction of chromosome,namely 1B monosomic,1B double ditelosomic (20Ⅱ+tt~(1BS)+tt~(1BL)) and nullitetrasomic (1B~01D~Ⅳ),and eight wheat varieties or lines and partial parent materials.Its haploid frequency of Male Sterile Lines and F_1,and the fertility restoration performance of F_1 were investigated.The results are as followings:① the frequency of the F_1 hybrid of the male sterile lines of 1B/1R wheat with Ae.bicornis cytoplasm was related to the constitution difference of 1B/1R translocation chromosome of male sterile lines;② it was also related to the construction of chromosome of male parent,and the aneuploid can increase the trendy of the frequency of haploid;③ there was no relation between the frequency of haploid and fertility;④ there is no haploid plant in the male sterile line of non-1B/1R wheat and its F_1.