Artificial Domestication Cultivation and Chemical Components Analysis of Ganoderma lucidum Strains from Belgrade

Short wood-logs (Quercus spp.) were applied in the artificial domestication cultivation of Ganoderma lucidum strains obtained from Belgrade University. The experiment result showed that strains GA-2 and GA-9 were the optimum strains with the yield being 8.8kg/m~3 and 8.3 kg/m~3 (dry fruitbody) respectively, and the average weight of single fruitbody being 12.38g and 12.16g (dry fruitbody) respectively. The microstructures of hymenium and basidiospores were observed by electric scanning microscopy (SEM). And the electrophoresis chromatogram of soluble mycoprotein of strains GA-2 and GA-9 by GC PAGE was basically identical. While the analytical results showed that the contents of water, crude protein, crude fibre, crude fat, polysaccharide and ash in the fruitbodies of G. lucidum GA-2 and GA-9 were 11.3% and 12.7%,11.6% and 12.8%, 28.7% and 30.8%, 4.8% and 5.6%,0.90% and 1.02%,1.4% and 1.7% respectively, that their fatty acids were mainly composed with the unsaturated fatty acids including oleic acid (C_(18:1),59.6%) and linoleic acid(C_(18:2),19.2%), and that 18 kinds of amino acids were found in the fruitbodies with a total content and E/T being 9.61% and 50.6% respectively.